F6401-01D
Frizzled 1 (FZD1)
Size
£576.00
- SKU:
- F6401-01D
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- NS0-derived recombinant mouse Frizzled-1 extracellular domain, Gln72-His248 (Met122Ile)
- Clone:
- 5A2
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.2, 5% trehalose. Reconstitute with 100ul sterile PBS.
- Extra Details:
- Members of the Frizzled family of proteins serve as receptors for the Wnt signaling pathway and are involved in a variety of developmental processes. Frizzled proteins each contain a divergent N-terminal signal peptide, a highly conserved extracellular cysteine-rich domain, a variable-length linker region, a seven-pass transmembrane domain and a variable-length C-terminal tail. Applications: Suitable for use in Western Blot and Direct ELISA. Other applications not tested. Recommended Dilution: Western Blot: Use at 1-2ug/ml with the appropriate secondary reagents to detect mouse Frizzled-1. Using a colorimetric detection system, the detection limit for rmFrizzled-1 is ~5ng/lane and 1ng/lane under non-reducing and reducing conditions, respectively. Direct ELISA: Used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect mouse Frizzled-1. The detection limit for rmFrizzled-1 is ~5ng/well. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice