F6401-01C

Frizzled 1 (FZD1)

Size

£863.00

SKU:: F6401-01C
Purity:: Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Recombinant protein corresponding to Gln72-His248 (Met122Ile) from mouse Frizzled-1 expressed in NS0 cells
Clone:: 5A1
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: Members of the Frizzled family of proteins serve as receptors for the Wnt signaling pathway and are involved in a variety of developmental processes. Frizzled proteins each contain a divergent N-terminal signal peptide, a highly conserved extracellular cysteine-rich domain, a variable-length linker region, a sevenpass transmembrane domain and a variable-length C-terminal tail. Applications: Suitable for use in Western Blot, Flow Cytometry and Direct ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml. Flow Cytometry: 0.25mg/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.