F6070-20
Fractin (Not for Export EU)
Size
£863.00
- SKU:
- F6070-20
- Purity:
- Serum
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to the last five amino acids, K-YELPD, of the C-terminus of the 32kD human Fractin fragment of beta-Actin. Species Sequence Homology: feline, porcine, sheep, bovine, canine, rabbit and guinea pig.
- Format:
- Supplied as a liquid. No preservative added.
- Extra Details:
- During apoptosis, beta actin is cleaved by activated caspase 3 between Asp244 and Gly245. This cleavage results in the release of an approximately 130 amino acid (15kD) fragment from the C-terminus of beta actin and a 32kD (244 amino acid) N-terminal fragment of actin, known as Fractin. The C-terminus of the Fractin fragment represents an apoptosis-specific neoepitope that is not antigenic in native full-length beta actin and therefore presents a novel and specific marker for distinguishing apoptotic from necrotic cells. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000-1:4000 for incubation overnight at 4°C. Recommended antibody dilution buffer: TBS with 3% BSA and 0.05% Tween-20, pH 7.4. Immunohistochemistry/Immunocytochemistry: 1:400-1:1000. Incubate 1 hr at 37°C or 4°C overnight. Paraffin-embedded tissues may require antigen retrieval methods. Optimal dilutions to be determined by the researcher. Positive Control: Staurosporin treated neuronal cultures Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice