F6070-17
Fractalkine (Neurotactin)
Size
£409.00
- SKU:
- F6070-17
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- E. coli-expressed chemotactic domain of rat fractalkine
- Format:
- Supplied as a lyophilized powder in 0.09% sodium azide.
- Extra Details:
- Fractalkine, also termed neurotactin, is a membrane-bound CX3C chemokine. The mature protein is part of a 397-amino acid precursor consisting of a chemokine domain (76 amino acids), a transmembrane domain (18 amino acids), and an intracellular tail of 37 amino acids. Within the chemokine domain the first two cysteine residues are separated by 3 amino acids. Fractalkine message is found at high concentrations in the brain, and also in kidney, lung and heart. Fractalkine is chemotactic for monocytes and may play a role in brain inflammation. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western blot: 1:1000-1:2000 Immunoprecipitation: 1:300-1:800 Immunohistochemistry: 1:100-1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice