Folate receptor 1 (FOLR1) (Folate receptor 1 (adult) Adult folate-binding protein, FBP, Folate receptor, adult, Folate receptor 1, Folate receptor alpha, FR-alpha, KB cells FBP, MOv18, Ovarian tumor-associated antigen MOv18)

Size

£889.00

SKU:: F5750-15B
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Recombinant protein corresponding to Arg25-Met233 of human FOLR1 expressed in CHO cells.
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: Folate receptor 1 (FOLR1; also folate receptor alpha, folate binding protein 1, and MOv18) is a 37-42kD member of the folate receptor family. FOLR1 is expressed in reticulocytes and placenta, plus renal, mammary and thymic epithelium, where it mediates the transport of 5-methyltetrahydrofolate into the cell. The human FOLR1 preproprecursor is 257aa in length. It codes for a GPI-linked glycoprotein that is 210 aa in size (aa25-234). There are two forms of FOLR1. One is GPI-linked, while the other is a soluble MMP cleavage product (aa25-226). There is one potential alternate splice form that shows a five aa substitution for aa 121-205. Over aa 25-233, human FOLR1 shares 83% aa identity with mouse FOLR1. Applications: Suitable for use in Flow Cytometry, ELISA and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 25ug/10e6 cells using MCF-1 cells Western Blot: 1ug/ml using HeLa cells Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice