F5250-17-HRP
Fluorescein Isothiocyanate (FITC) (HRP)
Size
£940.00
- SKU:
- F5250-17-HRP
- Purity:
- Purified by Protein A affinity chromatography. >90% tested by Immunoelectrophoresis.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- FITC-BSA conjugate.
- Clone:
- 1.BB.75
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with horseradish peroxidase (HRP).
- Extra Details:
- Fluorescein isothiocyanate (FITC) is a small fluoresent molecule that is commonly conjugated to antibodies for immunofluorescent cell labeling. In conjunction with anti-FITC antibodies, it can also be used as an epitope tag for detection and signal amplification. Applications: Suitable for use in the detection of FITC labeled antigens and antibodies, as a capture antibody for FITC labeled bioploymers, ELISA, and as a second enzyme for Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Hybridoma: Hybridization of SP2/0 myeloma cells with spleen cells from Balb/c mice. Affinity Constant: 1.5 x 10e-8 Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice