Fluorescein (Texas Red)

Size

£1,141.00

SKU:: F5200-31A
Purity:: Purified by immunoaffinity chromatography, pepsin digestion and chromatographic separation.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Fluorescein conjugated to goat IgG
Format:: Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Texas Red. Reconstitute with 1ml sterile ddH2O.
Extra Details:: Applications: Suitable for use in Immunomicroscopy, FLISA, Flow Cytometry and FACS. Other applications have not been tested. Recommended Dilution: Immunofluorescence Microscopy: 1:500-1:2500 Flow Cytometry: 1:2000-1:10,000 Optimal dilutions to be determined by the researcher. Label: Texas Red Sulfonyl Chloride (Molecular Weight 625D) Absorption Wavelength: 596nm Emission Wavelength: 620nm Fluorochrome/Protein Ratio: 3.5 moles Texas Red per mole of goat IgG Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.