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F5200-25

Fluorescein (HRP)

Size

£1,209.00

SKU:
F5200-25
Purity:
Purified by immunoaffinity chromatography.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Goat
Immunogen:
Fluorescein conjugated to goat IgG.
Format:
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA, 0.01% gentamicin sulfate. Reconstitute with 1ml sterile ddH2O. Labeled with horseradish peroxidase (HRP).
Extra Details:
Applications: Suitable for use in Western Blot, Dot Blot, ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: ELISA: 1:10,000-1:50,000 Western Blot: 1:2000- 1:5000 Immunohistochemistry: 1:500- 1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping Conditions:
Blue Ice