F4600-07
Flt3 Ligand (Fms-like Tyrosine Kinase 3 Ligand, flk2 Ligand)
Size
£466.00
- SKU:
- F4600-07
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to human Flt3 Ligand expressed in E. coli.
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
- Extra Details:
- Flt3-ligand (flk2-ligand), which stimulates the proliferation and colony formation of certain BM precursor cells including CD34+ cells, is a soluble 17.6kD polypeptide containing aa156 comprising the extracellular domain of the transmembrane flt3-ligand protein. Applications: Suitable for use in Neutralization, ELISA and Western Blot. Other applications have not been tested. Recommended Dilutions: Neutralization: 4-6ng/ml will yield one-half maximal inhibition [ND50] of the biological activity of human Flt3 Ligand (1ng/ml). ELISA (Sandwich): 0.5-2ug/ml using 100ul/well antibody solution in conjunction with biotylated anti-human Flt3 Ligand (F4600A) as a detection antibody, allows the detection of at least 2000-4000pg/well of recombinant human Flt3 Ligand. Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human Flt3 Ligand is 1.5-3ng/lane, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice