F4599-02

Flt1, ED (Fms-like Tyrosine Kinase 1, Vascular Endothelial Growth Factor Receptor 1, VEGF R1)

Size

£819.00

SKU:: F4599-02
Purity:: Purified by Protein A/G affinity chromatography from ascites.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to the extracellular domain of human FLt1. Actual epitope recognized by this clone is between aa1-250 of human Flt1.
Clone:: 2Q232
Format:: Supplied as a liquid in PBS, pH 7.4, 5% trehalose.
Extra Details:: Flt1 (human 1338aa, rat 1336aa and mouse 1330aa), a putative receptor protein tyrosine kinase, is a receptor for VEGF and PLGF. Flt1 has also been described as VEGFR1 and Flk-2. It is a type 1 membrane protein and is expressed in normal lung, placenta, liver, kidney, heart, and brain but not in tumor cells. Applications: Suitable for use in Western Blot, Flow Cytometry and ELISA. Other applications have not been tested. Recommended Dilutions: Western Blot: 0.5-2.0ug/ml Flow Cytometry: 25ug/ml. Use 10-20ul to label 1-2x10e5 cells in a total volume of 200-250ul. Visualize with appropriate secondary antibodies. ELISA: 1:10,000-1:100,000 using 50-10ng of control peptide per well Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.