F4568-01B

Flotillin 1 (FLOT1, Reggie 2)

Size

£642.00

SKU:: F4568-01B
Purity:: Purified by peptide affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to C-SISQVNHKPLRTA, from the C Terminus of human Flotillin 1 (NP_005794.1).
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined. Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. Recommended Dilutions: Peptide ELISA Titer: 1:32,000 Western Blot: 0.3-1ug/ml ~48kD band observed in lysates of cell line H460 (calculated MW of 47.4kD according to NP_005794.1). In transfected HEK293 transiently expressing FLOT1 a band of approx. 48kD is observed. This band is not observed in the non-transfected HEK293. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.