FLIP, NT (FLICE Inhibitory Protein)

Size

£655.00

SKU:: F4550-05
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to 17 amino acids near the N-terminus of human FLIP.
Format:: Supplied as a liquid in PBS, 0.02% sodium azide.
Extra Details:: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively1,2. The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH and CLARP3-7. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and –10, and potently inhibits apoptosis induced by all known death receptors. Four splice variants of c-FLIPs have been identified and termed FLIPa, b, g, and d, respectively. Applications: Suitable for use in Flow Cytometry, ELISA, Immunofluorescence, Immunohistochemistry, Western Blot and Immunocytochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: 1-2ug/ml Immunohistochemistry (paraffin): 5ug/ml Immunocytochemistry: 5ug/ml Immunofluorescence: 20ug/ml Optimal dilutions to be determined by the researcher. Positive Controls: HeLa Cell Lysate Mouse Liver Tissue Lysate Jurkat Cell Lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice