FLASH, CT (FLICE-associated Huge Protein)

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£655.00

SKU:: F4538
Purity:: Immunoaffinity chromatography purified IgG
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Raised against a synthetic peptide (SERFQQLMKLFEKSKC) corresponding to amino acids 1253 to 1268 of human origin, which differ from those of mouse by one amino acid (1,2).
Format:: Supplied as a liquid, 100ug in 200ul of PBS, 0.02% sodium azide.
Extra Details:: A novel mammalian CED-4 homologous was recently identified and cloned in mouse (1) and human (2) and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8 (1). There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity (1,2). FLASH is widely expressed. Applications: Suitable for use in ELISA, Western Blotting. Other applications not tested. Recommended Dilution: Western Blotting: 0.25-1ug/ml. Optimal dilutions to be determined by the researcher. Positive Control: HeLa whole cell lysate Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice