Flag (DYKDDDDK) Epitope Tag

Size

£505.00

SKU:: F4524-72L
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Chicken/Avian
Immunogen:: Flag (DYKDDDDK) Epitope Tag, conjugated to KLH.
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Extra Details:: The DYKDDDDK peptide is a small component of the epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. Applications: Suitable for use in ELISA, Western Blot and Immunofluorescence/Immunocytochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:5000-1:10,000 Western Blot: 1:2000-1:10,000 Immunofluorescence (IC): 1:500 Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1906-10: IgY, Chicken-HRP Rb x Ch I1906-12: IgY, Chicken-FITC Rb x Ch I1906-14: IgY, Chicken-AP Rb x Ch Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.