Flag (DYKDDDDK) Epitope Tag (HRP)

Size

£895.00

SKU:: F4524-72H
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to Enterokinase cleavage site, DYKDDDDK, conjugated to KLH. Residues of cysteine and glycine were used to facilitate coupling at the C-terminal end.
Format:: Supplied as a lyophilized powder from PBS, pH 7.2, 1% BSA., 0.01% gentamicin sulfate. Reconstitute with 100ul sterile ddH2O. Labeled with Horseradish peroxidase (HRP).
Extra Details:: Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 1:10,000-1:50,000 Western Blot: 1:1000-1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.