Flag (DYKDDDDK) Epitope Tag

Size

£354.00

SKU:: F4524-72A
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH.
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 0.02% sodium azide. Reconstitute with 80ul sterile ddH2O.
Extra Details:: The DYKDDDDK peptide is a small component of the epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry. Other applications not tested. Recommended Dilutions: ELISA: 0.01-0.05ug/ml Western Blot: 0.1-1ug/ml Flow Cytometry: 1-3ug Labels 1x10e6 cells Immunofluorescence: 5-20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.