Fibronectin (Biotin)

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£1,081.00

SKU:: F4215-46-BIOTIN
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Fibronectin extracted and purified from mouse plasma.
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Extra Details:: Fibronectin is a high molecular weight extracellular adhesion molecule involved in many cellular processes, including tissue repair or wound healing, embryogenesis, blood clotting, and cell migration/adhesion. Fibronectin exists in two major forms: 1) as an insoluble glycoprotein dimer that serves as a linker in the extracellular matrix (ECM), and 2) as a soluble disulfide-linked dimer found in the plasma. The plasma form is synthesized by hepatocytes, and the ECM form is made by fibroblasts, chondrocytes, endothelial cells, macrophages, as well as certain epithelial cells. Fibronectin sometimes serves as a general cell adhesion molecule by anchoring cells to collagen or proteoglycan substrates. It can also serve to organize cellular interaction with the ECM by binding to different components of the ECM and to membrane- bound fibronectin receptors on cell surfaces. Applications: Suitable for use in Immunohistochemistry, RIA, ELISA, Immunofluorescence and Immunocytochemistry. Other applications have not been tested. Recommended Dilution: Immunohistochemistry: 1:80 for immunofluorescent staining of frozen mouse skin and liver tissues. Immunofluorescence (IC): Evaluated on NIH/3T3 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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