F4215-30
Fibronectin (Biotin)
Size
£896.00
- SKU:
- F4215-30
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Fibronectin purified from human plasma.
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin. Reconstitute with 100ul sterile ddH2O.
- Extra Details:
- Fibronectins are disulfide-linked, dimeric glycoproteins of ~440kD. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectins are involved in cell adhesion, tissue organization and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors. Applications: Suitable for use in ELISA, Immunoprecipitaion, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 1:4000-1:20,000. Assayed against 1ug of Fibronectin in a standard capture ELISA using ABTS (A0030) as a substrate for 30 minutes at RT. Immunohistochemistry (Paraffin embedded tissue): 1:50-1:200 Western Blot: 1:500-1:10,000 Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher. Biotin/Protein Ratio: 10-20 BAC molecules per rabbit IgG molecule. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice