Fibronectin (FN, Cold-insoluble Globulin, CIG, FN1) (Biotin)

Size

£1,106.00

SKU:: F4215-13A
Application:: WB
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: Purified, human plasma-derived, Fibronectin. Species sequence homology: bovine (92%), mouse (90%).
Format:: Supplied as a lyophilized powder from PBS, 50ug/1ug BSA. Labeled with Biotin. Reconstitute with sterile TBS, pH 7.3, 20mM Trizma, 150mM sodium chloride, 0.1% BSA.
Extra Details:: Plasma Fibronectin is a secreted dimeric protein. It is involved in cell adhesion, migration, wound healing and other cellular processes. Human Fibronectin shares 92% and 90% aa sequence homology with bovine and mouse Fibronectin, respectively. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 0.1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile TBS, 0.01% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.