F4212-97

Fibroblast Growth Factor Binding Protein (FGFBP, FGF-BP, 17kD Heparin-Binding Growth Factor Binding Protein, 17kD HBGF Binding Protein, HBp17)

Size

£876.00

SKU:: F4212-97
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Recombinant protein corresponding to Lys34-Cys234 from human FGF-BP expressed in E. coli.
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: FGF-BP, also known as 17kD heparin-binding growth factor binding protein (HBp17) is a secreted, low affinity heparin binding protein. It binds reversibly to FGF-1, -2, -7, -10, and -22 and functions to release the FGFs from the extracellular matrix for interactions with FGF receptors. Human FGF-BP shares 66% amino acid sequence homology with the mouse protein. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 0.1ug/ml. The detection limit is ~1ng/lane under non-reducing and reducing conditions. ELISA (Direct): 0.5-1ug/ml. The detection limit is ~0.5ng/well. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.