F4212-20

FGF15 (Fibroblast Growth Factor 15, FGF-15)

Size

£856.00

SKU:: F4212-20
Application:: IHC-Fr
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: Recombinant protein corresponding to aa24-218 from mouse FGF-15 expressed in E.coli (O35622)
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: FGF-15 is a 25kD, secreted, nonglycosylated polypeptide that is a member of the FGF-15/21/23 subfamily, FGF family of growth factors. It is synthesized by ileal epithelium, colonic myofibroblasts, and select areas of the developing brain. FGF-15 acts on hepatocytes and reduces CYP7A1 levels, thus blocking bile acid production. It also promotes colonic epithelial migration, and in the embryonic brain, inhibits over-production of neuronal precursors. FGF-15 is synthesized as a 218aa precursor that contains a 25aa signal sequence and a 182aa mature region aa26-218; 23kD predicted MW). Mature FGF-15 contains a characteristic 3-dimensional B-trefoil structure, plus a unique Cys pattern that contributes to stability. There are two potential splice forms. One shows a 23aa substitution for aa120-218, while a second shows a 41aa substitution for aa120-218. Over aa23-200, mouse FGF-15 shares 96% aa identity with rat FGF-15. Although mouse FGF-15 is considered the ortholog to human FGF-19, the two molecules share only 54% aa identity over aa23-200, and are produced by different cell types (intestinal epithelium in mouse, hepatocytes in human). Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: 5-15ug/ml using immersion fixed frozen sections of monkey embryo Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice