F4211-75E
Fibroblast Growth Factor 9 (FGF9)
Size
£639.00
- SKU:
- F4211-75E
- Purity:
- Purified by Immunoaffinity chromatography. Endotoxin: ≤1EU/ug.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to mouse FGF-9, expressed in E. coli.
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O to a concentration of 0.1-1.0mg/ml.
- Extra Details:
- FGF-9 is a heparin binding growth factor that belongs to the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-9 targets glial cells, astrocytes cells and other cells that express the FGFR 1c, 2c, 3b, 3c and 4. Applications: Suitable for use in ELISA, Western Blot, and Neutralization. Other applications have not been tested. Recommended Dilutions: ELISA (Sandwich): 0.5-2.0ug/ml using 100ul/well antibody solution) In conjunction with biotinylated anti-mouse FGF9 (F4211-75B) as a detection antibody, allows the detection of at least 0.2-0.4ng/well of recombinant human mouse FGF9. Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mouse FGF9 is 1.5-3ng/lane, under either reducing or non-reducing conditions. Neutralization: 0.2ug/ml yields one-half maximal inhibition (ND50) of the biological activity of mouse FGF9 (23.3ng/ml). Optimal working dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice