F4211-50
Fibroblast Growth Factor 8, Recombinant, Human (FGF8)
Size
£301.00
- SKU:
- F4211-50
- Molecular Weight:
- 22.4
- Purity:
- ≥ 95% by SDS-PAGE and HPLC. Endotoxin: ≤ 0.1ng/ug (1EU/ug).
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Format:
- Supplied as a lyophilized powder from 10mM Tris, pH 8.0, 75mM sodium chloride. Reconstitute with 10mM Tris, pH 8.0 to a concentration of 0.1-1.0mg/ml. Do not vortex. Further dilute for extended storage with a buffer containing a carrier protein (0.1% BSA or HSA).
- Extra Details:
- Fibroblast Growth Factor-8 (FGF-8b) is a heparin binding growth factor belonging to the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. There are 4 known alternate spliced forms of FGF8: FGF8A, FGF-8B, FGF-8E and FGF-8F. The human and murine FGF8 A and B are identical. Human and murine FGF8 E and F are 98% identical. FGF-8 targets mammary carcinoma cells and other cells expressing the FGF receptors. Recombinant human FGF-8b is a 22.4kD protein consisting of 193 amino acid residues. Biological Activity: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors is ≤ 0.5ng/ml, corresponding to a specific activity of ≥ 2x10e6 units/mg Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice