F4210-10A
Fibroblast Growth Factor, Acidic, Recombinant, Human (FGFa)
Size
£364.00
- SKU:
- F4210-10A
- Molecular Weight:
- 15.967
- Purity:
- ≥ 95% (HPLC and SDS-PAGE). Endotoxin: ≤ 0.1ng/ug (<1EU/ug)
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Format:
- Supplied as a powder lyophilized in PBS, pH 7.5. Reconstitute with sterile dH2O to 0.1mg/ml, which can be further diluted into other aqueous solutions. Do not vortex.
- Extra Details:
- FGF-acidic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-acidic is a non-glycosylated heparin binding growth factor that is expressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. FGF-acidic has the ability to signal through all the FGF receptors. Recombinant Human FGF-acidic is a 16.8kD protein consisting of 141 amino acid residues. Recombinant protein corresponding to human FGF-acidic, expressed in E. coli. Amino Acid Sequence: MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D Biological Activity: Determined by a cell proliferation assay using Balb/c 3T3 cells. The expected ED50 is ≤0.5ng/ml in the presence of 10ug/ml heparin, corresponding to a specific activity of ≥ 2x10e6 units/mg. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Ambient