F4208-57E-PE
Fibroblast Activation Protein alpha (FAPa, FAP, EC 3.4.21.-, Seprase) (PE)
Size
£856.00
- SKU:
- F4208-57E-PE
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Leu26-Asp760 of human Fibroblast Activation Protein alpha, expressed in Sf21 cells.
- Clone:
- 9H122
- Format:
- Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
- Extra Details:
- FAP (Fibroblast Activation Protein alpha; also known as seprase) is a 97kD Type II transmembrane serine protease. FAP degrades gelatin, Type I collagen and N-terminal prolines. Enzymatically active FAP is a dimer that migrates at ~170kD. FAP is induced on reactive stromal fibroblasts in epithelial cancers, sarcomas and granulation tissue, and may play roles in tumor invasion, tissue remodeling and wound repair. The 760 amino acid (aa) human FAP contains a 735aa extracellular domain that shares 90% aa identity with mouse or rat FAP. A reported 672aa splicing variant diverges prior to the active site charge relay residues at the C-terminus. Applications: Suitable for use in Flow Cytometry. Other applications have not been tested. Recommended Dilutions: Flow Cytometry: 10ul labels 10e6 cells in WI‑38 human lung fibroblast cells and U87MG cells. Optimal dilutions to be determined by researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice