F4200-07B

Fibrinogen (Human Plasma) (Biotin)

Size

£889.00

SKU:: F4200-07B
Purity:: Purified by delipidation, salt fractionation and ion exchange chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Fibrinogen from human plasma
Format:: Supplied as a lyophilized power from PBS, pH 7.2, 10mg/ml BSA, 0.09% sodium azide. Labeled with Biotin. Reconstitute with 100ul ddH2O.
Extra Details:: Fibrinogen is an abundant plasma protein (5-10uM) produced in the liver. The intact protein has a molecular weight of 340kD and is composed of 3 pairs of disulfide-bound polypeptide chains named Aalpha, Bbeta and gamma. Fibrinogen is a triglobular protein consisting of a central E domain and terminal D domains. Proteolysis by thrombin results in release of Fibrinopeptide A (FPA, Aa1-16) followed by Fibrinopeptide B (FPB, Bb1-14) and the fibrin monomers that result polymerize in a half-overlap fashion to form insoluble fibrin fibrils. The chains of fibrin are referred to as a, b and g, due to the removal of FPA and FPB. The polymerized fibrin is subsequently stabilized by the transglutaminase activated Factor XIII that forms amide linkages between g chains and, to a lesser extent, a chains of the fibrin molecules. Proteolysis of fibrinogen by plasmin initially liberates C-terminal residues from the Aa chain to produce fragment X (intact DE- D, which is still clottable). Fragment X is further degraded to nonclottable fragments Y (D-E) and D. Fragment Y can be digested into its constituent D and E fragments. Digestion of non-crosslinked fibrin with plasmin is very similar to the digestion of fibrinogen, which results in production of fragments D and E. Degradation of crosslinked fibrin by plasmin results in fragment DD (D-Dimer consisting of the D domains of 2 fibrin molecules crosslinked via the y chains), fragment E (central E domain) as well as DDE in which fragment E is non-covalently associated with DD. For human crosslinked fibrin, the relative weights of the cleavage fragments produced are: 184kD for fragment DD, 92kD for D, 50kD for E, 1.54kD for FPA and 1.57kD for FPB. Applications: Suitable for Immunoblot (Western or Dot Blot), ELISA, Immunomicroscopy and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:50,000-1:250,000; 1ug of Fibrinogen was used in a standard capture ELISA with Streptavidin (HRP), Cat # S7973-97A, and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]), Cat # A0030 as a substrate for 30 minutes at RT. Optimal dilutions to be determined by the researcher. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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