F4199-92J
Fibrinogen (Coagulation Factor I) (FITC)
Size
£594.00
- SKU:
- F4199-92J
- Application:
- FLISA
- Purity:
- Purified by DEAE chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Fibrinogen isolated from fresh rat plasma after removing prothrombin
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC). Reconstitute in 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
- Extra Details:
- Fibrinogen (clotting factor I) is a heat labile beta glycoprotein present in plasma. It is the precursor of fibrin, which is the key protein constituting the network of the blood clot. Thrombin converts fibrinogen to fibrin by limited proteolysis. Fibrin monomers polymerize to fibrin which is stabilized by cross-linking. Applications: Suitable for use in FLISA, Immunoelectrophoresis and Radial Immunodiffusion. Other applications not tested. Recommended Dilution: FLISA: 1:10-1:40 Immunofluorescence: 1:10-1:40 Optimal dilutions to be determined by the researcher. Fluorescein/IgG protein molar ratio (F/P): ~2.2 Excitation: 492nm Emission: 515nm Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
- Shipping Conditions:
- Blue Ice