F4199-92
Fibrinogen (Coagulation Factor I)
Size
£561.00
- SKU:
- F4199-92
- Purity:
- Purified by Protein A affinity chromatography from ascites
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Fibrin degradation products
- Clone:
- 5E598
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide
- Extra Details:
- Fibrinogen is synthesized in the liver and platelets and plays a major role in the coagulation process. It consists of 6 polypeptide chains, two each of A alpha-, B beta- and gamma-chains and is a dimeric molecule, each subunit consisting of one of each chain type. Thrombin cleaves N-terminal peptides from each of the A alpha-and B beta-chains to form fibrin monomer. The newly exposed N-terminal sites spontaneously link to C-terminal sites of another fibrin molecule to form a fibrin chain. These are then crosslinked by activated Factor XIII which stabilizes the fibrin clot. Normal plasma levels of fibrinogen are around 2.6g/L. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Recommended Pair: Capture: F4205-99 Detection: F4199-92 Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice