Fibrinogen (Coagulation Factor I)

Size

£636.00

SKU:: F4199-91E
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Fibrinogen isolated from fresh plasma after removing prothrombin
Format:: Supplied as a lyophilized powder from PBS, pH 7.2, No preservative added. Reconstitute with 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
Extra Details:: Fibrinogen (clotting factor I) is a heat labile beta glycoprotein present in plasma. It is the precursor of fibrin, which is the key protein constituting the network of the blood clot. Thrombin converts fibrinogen to fibrin by limited proteolysis. Fibrin monomers polymerize to fibrin which is stabilized by cross-linking. Applications: Suitable for use in ELISA, Immunoelectrophoresis and Radial Immunodiffusion (Ouchterlony). Other applications not tested. Recommended Dilution: ELISA: 1:500-1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.