F4197-23-BIOTIN

Fibrin Degradation Product, D-Dimer (Biotin)

Size

£1,081.00

SKU:: F4197-23-BIOTIN
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Homogenized fibrin clot.
Clone:: 1.B.677
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with Biotin.
Extra Details:: Human D-dimer (DD), a specific degradation product of cross-linked fibrin, is released into the bloodstream during clot breakdown. It is used as an indicator of blood clots, venous thromboses or pulmonary embolisms. Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. Recommended Dilutions: Sandwich ELISA: Plasma samples should be diluted with 10mM Tris-HCl buffer, pH 7.5, 1M sodium chloride, 0.1% Tween-20. To avoid non-specific binding, the final sodium chloride concentration in plasma samples should be ≥0.5M. Optimal dilutions to be determined by the researcher. Hydridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.