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F4197-19-FITC

Fibrin Degradation Product, D-Dimer (FITC)

Size

£1,081.00

SKU:
F4197-19-FITC
Purity:
Purified by Protein A affinity chromatography (>90% SDS-PAGE).
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Mouse
Immunogen:
Homogenized fibrin clot
Clone:
1.B.673
Format:
Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with Fluorescein isothiocyanate (FITC).
Extra Details:
Human D-dimer (DD), a specific degradation product of cross-linked fibrin, is released into the bloodstream during clot breakdown. It is used as an indicator of blood clots, venous thromboses or pulmonary embolisms. Applications: Suitable for use in FLISA and Western Blot. Other applications have not been tested. Recommended Dilution: Sandwich FLISA: Plasma samples should be diluted with 10mM Tris-HCl buffer, pH 7.5, 1M sodium chloride, 0.1% Tween 20. To avoid non-specific binding, the final sodium chloride concentration in plasma samples should be ≥ 0.5M. Optimal dilutions to be determined by the researcher. Matched Pair: Suitable for use as the capture or solid phase antibody. Capture: F4197-19 Matched to F4197-17, F4197-18 or F4197-20 as the detection or conjugate (label) antibody. Hydridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
Shipping Conditions:
Blue Ice