F4150-01
FK506 Binding Protein (FKBP59, HSP56, p59)
Size
£563.00
- SKU:
- F4150-01
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Partially purified 9S rabbit uterine progesterone receptor
- Clone:
- 4E83 (KN382/EC1)
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
- Extra Details:
- The 59kD FK506 Binding Protein, FKBP59, was initially characterized as a component of steroid receptor complexes (1). FKBP59 has been identified as a member of the Hsp90/Hsp70 steroid receptor complex, which has been used to differentiate between the active and inactive forms of steroid receptors (2). FKBP59 is alternatively termed FKBP52, HBI and Hsp56 in literature. FKBP59 is an immunophilin of the FK506 binding type whose cellular function is not fully known, but is thought to play a role in the folding and cytoplasmic/nuclear distribution of steroid receptors (3). FKBP59 (Hsp56) exhibits stress protein properties and has been shown to have increased expression in response to heat shock and ischemia (4). Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Western Blot (Colorimetric): 2ug/ml. Detects ~59kD band. Additional unidentified 50kD band observed in some cell lysates. Immunoprecipitation: 10ug/ml Immunohistochemistry: 1:3000 Optimal dilutions to be determined by researcher. Positive Controls: HeLa Heat Shocked Cell Lysate Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice