FHL1 (Four and a Half LIM Domains Protein 1, FHL-1, Skeletal Muscle LIM-protein 1, SLIM, SLIM-1, SLIM1)

Size

£642.00

SKU:: F4149-77J
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to CNKRFVFHNEQVY, from human FHL1, at the internal region. Species sequence homology: Bovine, canine, porcine and rat.
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: FHL1 is a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. Expression of these family members occurs in a cell- and tissue-specific mode and these proteins are involved in many cellular processes. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:32,000 Western Blot: 0.05-0.3ug/ml, observed in human muscle lysates on ~30kD bands Immunohistochemistry (Paraffin): 2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.