FHL1 (Four and a Half LIM Domains 1, FHL-1, FHL1A, FHL1B, FLH1A, KyoT, LIM Protein SLIMMER, RBP Associated Molecule 14-1, RAM14-1, Skeletal Muscle LIM Protein 1, SLIM1, SLIM-1, SLIM, XMPMA)

Size

£837.00

SKU:: F4149-77E1
Application:: WB
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Recombinant corresponding to Thr93-Asn166 from human FHL1 expressed in E. coli.
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: FHL1 is a 36kD member of the four-and-a half class of the LIM domain-only protein family. It is highly expressed in myocardium and skeletal muscle, and is now known to bind NFATc1, promoting muscle hypertrophy. It is also noted to be a RIP140 binding partner that inhibits estrogen receptor signaling. Human FHL1 is 323aa in length. It contains one C4-type Zn-finger region (aa7-31), three distinct LIM domains (aa40-77; 101-158; 162-212) and a bipartite NLS (aa231-246). There is one 32kD splice variant that shows a 50 aa substitution for aa231-323. This creates a fourth LIM domain. There are also alternative start sites aa29-16 upstream of the standard site, a deletion of aa168-296, and an 11 aa substitution for aa81-91. Over aa93-166, human FHL1 is 97aa identical to mouse FHL1. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice