F2954-16D
FAM3D (Family with Sequence Similarity 3 Member D, EF-7)
Size
£832.00
- SKU:
- F2954-16D
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Trp39-Phe224 from human FAM3D, expressed in NSO cells (NP_620160).
- Clone:
- 7H133
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Family with sequence similarity 3, member D (FAM3D), also known as EF-7, belongs to the novel cytokine-like family whose members are secreted four-helix bundle proteins. Human FAM3D is abundantly expressed in placenta as a 224 amino acid (aa) residue precursor with a 25aa signal sequence. The aa sequence of mature human FAM3D is 74% identical to that of mouse FAM3D. Applications: Suitable for use in Western Blot and Direct ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1-2ug/ml. The detection limit for recombinant human FAM3D is ~50ng/lane under non-reducing and reducing conditions. Direct ELISA: 0.5-1ug/ml. The detection limit for recombinant human FAM3D is ~13ng/well. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice