F0025-73A

FCRLB, aa18-427, Recombinant, Mouse (FCRL2, FCRY, FREB2, Fc Receptor-like B, Fc Receptor-like and Mucin-like Protein 2, Fc Receptor-like Protein 2, Fc Receptor-related Protein Y)

Size

£863.00

SKU:: F0025-73A
Molecular Weight:: 46.5
Purity:: ~80% (SDS-PAGE). Endotoxin: ≤0.011EU/ug (LAL).
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder in PBS. Reconstitute with 500ul sterile PBS.
Extra Details:: FCRLB, is a 60kD protein with sequence homology to classical Fc receptors. There are at least six type 1 transmembrane FCRL proteins and two that lack transmembrane segments. Each family member contains between three and nine immunoglobulin-like domains. FCRL proteins are differentially expressed within the B cell lineage and can either promote or inhibit B cell proliferation and activation (1, 2). According to R&D Systems testing, FCRLB binds to purified mouse IgG. Mouse FCRLB shares 82%, 94% aa sequence identity with human and rat FCRLB, respectively. It contains a putative signal peptide, three immunoglobulin-like domains, and a mucin-like stalk that is rich in Pro, Ser, and Thr residues (1-3). When expressed in transfectants, FCRLB is not secreted but shows a diffuse intracellular localization (4, 5). FCRLB is expressed at a low level in placenta and in B lineage cells of the germinal center (4, 5). It is up-regulated in B cells by BAFF and LPS (3). Among non-hematopoietic cells, FCRLB is expressed in fibroblasts, melanocytes, and melanoma (3, 4, 6). It is preferentially expressed in non-proliferating cells and at the onset of apoptosis (3, 5). Source: Recombinant corresponding to aa18-427 from mouse FCRLB, fused with 6-His tag at C-terminal, expressed in myeloma cell line, NSO. Molecular Weight: ~46.5kD Biological Activity: Measured by its ability to bind mouse IgG with an estimated kD<300nm. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice