F0020-11B

FASN (Fatty Acid Synthase) (Not for Export EU)

Size

£594.00

SKU:: F0020-11B
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Internal synthetic peptide corresponding to the N-terminus of mouse FASN (KLH). Cellular Localization: Cytoplasmic
Format:: Supplied as a liquid, 0.05% sodium azide.
Extra Details:: FAS (Fatty Acid Synthase) is a central enzyme in de novo lipogenesis (triglyceride production). It is a target for SREBP and is upregulated by LXR activation. Fatty liver is often the result of FAS activation. It is also one of the accepted markers for insulin resistance and SREBP, LXR activation. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 A band should be seen at ~272kD on SDS-PAGE. May see 1 or 2 minor cross-reacting lower MW bands in liver tissue. Immunoprecipitation: 1:100 Immunohistochemistry: Paraffin Optimal dilutions to be determined by the researcher. Positive Control: Mouse liver lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.