F0019-77

Fatty Acid Binding Protein, Heart, Adipocyte (FABP) (Not for Export EU)

Size

£704.00

SKU:: F0019-77
Purity:: Serum
Storage Conditions:: 4°C (-20°C Glycerol)
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: 12aa synthetic peptide corresponding to the C-terminus of human FABP (KLH).
Format:: Supplied as a liquid in 0.09% sodium azide, 40% glycerol.
Extra Details:: Fatty acids are important for general cellular metabolism. A number of proteins have been implicated in the transport and storage of fatty acid. FABPs (fatty acid binding proteins) are a group of cytoplasmic, small mol wt (14-15kD). FABP’s have a widespread tissue distribution. FABPs are quite abundant (3-5% of total cellular protein). At least 7 FABPs, FABP1-7, have been cloned and characterized from various tissues. FABPs can bind long-chain fatty acid, fatty-acid acyl-CoA and acyl-L-carnitine. Several different isoforms of FABP have been identified. These isoforms are generally referred to by tissue type (liver, heart, intestine, adipocyte, kidney, brain, etc. For example, protein designated as H-FABP indicates that it is heart type). Expression of these isoforms is not exclusive. More than one isoform can be found in a given cell or tissue. Three main types of FABPs that were initially discovered in the heart (FABP-H), liver (FABP-L), and intestine (FABP-I) are not exclusive to these tissues and show considerable differences at the amino acid level (~30% identity). Other FABPs detected in adipocyte, kidney, and brain show a high degree of sequence homology among each other and with other FABPs. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000. ELISA: 1:1,000-1:10,000. Coat with 5-10ug F0019-77E. Optimal dilutions to be determined by the researcher. Control Peptides: F0019-77E: Fatty Acid Binding Protein, Heart, Adipocyte, Human (FABP), Control Peptide F0019-77M: Fatty Acid Binding Protein, Human, Heart, Adipocyte, Human (FABP) Western Blot Control Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice