F0019-68

Fatty Acid Amide Hydrolase (FAAH)

Size

£754.00

SKU:: F0019-68
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: 17aa peptide sequence near the N-terminal of human FAAH (KLH).. Species Sequence Homology: rat (100%), mouse and porcine (94%), chicken (58%).
Format:: Supplied as a liquid in PBS, 0.1% BSA.
Extra Details:: Cannabinoids, a group of C21 compounds present in Cannabis sativa L., their carboxylic acids, analogs, and transformation products, are the active ingredients found in hasish and marihuana. (-)-trans-D9-tetrahydrocannabinol (D9-THC) is the major psychopharmacologically active component of Cannabis. Cannabis affects cognition and memory, euphoria and sedation, and antinociception (analgesia) without the respiratory depression problems associated with opioid analgesics. To date, two sub-types of the G-protein coupled cannabinoid receptor, CB1 and CB2, have been identified. The first brain-derived endogenous cannabinoids, an unsaturated fatty-acid ethanolamide, arachidonylethanolamide (AEA, also called anandamide) was found in brain. AEA has higher affinity for the CB1 than for the CB2. Neurons and astrocytes have been found to re-uptake and hydrolyse anandamide rapidly, resulting in the formation of arachidonic acid and ethanolamine. The uptake mechanism has been shown to be mediated by a saturable, selective, temperature-dependent and Na+-independent transporter. Anandamide hydrolysis is catalyzed by a membrane-bound amidohydrolase (called anandamide amidohydrolase or fatty acid amide hydrolase, FAAH). FAAH (rat/mouse/human 579aa; chromosome 1p34-p35; mol wt ~67 kD) sequence analyses suggest a single predicted transmembrane domain at the extreme N-terminus of the enzyme. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: ELISA: 1:10,000-1:50,000 using 50-100ng F0019-68A control peptide/well. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. .

Shipping Conditions:: Blue Ice