CD178 (sFas Ligand, Apoptosis Antigen Ligand, APT1LG1, APTL, CD95 Ligand, CD95L, CD95-L, FAS Antigen Ligand, Fas Ligand, FASL, FASLG, Tumor Necrosis Factor Ligand Superfamily Member 6, TNFSF6) (Biotin)

Size

£479.00

SKU:: F0019-65T3
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Highly pure recombinant human FAS Ligand, expressed in CHO cells.
Format:: Supplied as a lyophilized powder from PBS, pH 7.4. Reconstitute with sterile dH2O. Labeled with Biotin.
Extra Details:: Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: To detect hFasL/Apo1L by direct ELISA (using 100ul/well antibody solution) this antibody can be used at a concentration of 0.15-0.30 mg/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2ng/well of recombinant hFasL/Apo1L. Western Blot: To detect hFasL/Apo1L by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 mg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFasL/Apo1L is 1.5-3.0ng/lane, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.