F0019-64
Fas, Neutralizing (CD95, APO-1)
Size
£971.00
- SKU:
- F0019-64
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human Fas.
- Clone:
- 0.T.46 (ZB4)
- Format:
- Supplied as a liquid in PBS, pH 7.2, 50% glycerol.
- Extra Details:
- Fas (also known as Apo-1 and CD95) is a 40-50kD cell surface glycoprotein and a member of the TNF receptor family. Fas is an apotosis signalling molecule that forms the death inducing signalling complex (DISC) upon binding by the Fas ligand trimer on an adjacent cell. The Fas receptor also trimerizes, after which it is internalized and can then bind to the adaptor molecule FADD. FADD facilitates binding of caspase-8. which is activated to induce caspase-8 mediated apoptosis. Fas also plays a role in T cell mediated cytotoxicity and T cell development. Fas is found in the thymus, liver, heart, and ovary, where it is expressed on a minority of resting T and B cells and monocytes. Applications: Suitable for use in Neutralization, Flow Cytometry and Western Blot. Other applications have not been tested. Recommended Dilutions: Neutralization: 10-500ng/ml of this antibody was incubated for one hour with human Fas- transfected cells and inhibited apoptosis induced by the anti-Fas (human, activating) clone CH11/clone 0.T.45 (Catalog # F0019-63) treatment of the cells. Cell viability was assessed using a WST-1 assay. Western Blot: 5ug/ml Flow Cytometry: 5-20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice