Fas (CD95, APO-1)

Size

£960.00

SKU:: F0019-63D
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to a portion of mouse Fas.
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide
Extra Details:: Fas/APO-1/CD95 is a 36kD cell surface type I-membrane glycoprotein with an apparent molecular weight of 44kD on SDS-PAGE (1). Fas is a member of the tumor necrosis factor receptor (TNFR) family, which includes TNFR1, TNFR2, CD27, CD30 and CD40. Binding of Fas-ligand (FasL) to Fas or crosslinking of Fas by anti-Fas monoclonal antibodies leads to apoptosis of Fas expressing cells (2,3). Both Fas and TNFR contain a 70 amino acids cytoplasmic ìdeath domainî that is responsible for transmitting signal for apoptosis (4). Apoptosis induced by Fas-Fas ligand interaction is inhibited by a number of cytosolic factors such as the ICE/CED3 family inhibitor CrmA, and the short and long form of FLIP (5,6). Insertion of an early transposable element in intron 2 of Fas structural gene, as in the mouse lpr mutations, causes lymphoproliferation and autoimmunity in mutant mice, suggesting that Fas is involved in clonal deletion of autoreactive T cells. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot (ECL): 1:1000 Immunohistochemistry: 1:50 Optimal dilutions to be determined by researcher. Positive Control: Rat Brain Tissue Extract Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice