F0019-62

Fas (CD95, APO-1)

Size

£825.00

SKU:: F0019-62
Purity:: Purified by Protein G affinity chromatography from culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Recombinant protein containing the cytoplasmic domain of mouse Fas
Clone:: 0.T.44 (7C10)
Format:: Supplied as liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide
Extra Details:: Apoptosis or programmed-cell death is a physiological process essential for the normal development and maintenance of homeostasis in many organisms. This “cellular suicide” can be mediated by the Fas antigen (CD95, APO1), a cell-surface glycoprotein, 40-50kD, that belongs to the nerve growth factor/tumor necrosis factor (TNF) receptor family. Mice carrying the lymphoproliferation (lpr) mutation, which develop lymphoadenopathy and suffer from a systematic lupus erythematosus-like autoimmune disease, have defects in the Fas gene. Fas is expressed in several tissues including blood, where it expression is upregulated on activated T and B lymphocytes and natural killer cells. Binding of the Fas antigen by agonistic anti-Fas antibody has been shown to induce apoptosis. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml detects Fas (~45 kD) in RIPA lysates of mouse 3T3/A31 fibroblast cells and bovine brain. Immunoprecipitation: 4ug immunoprecipitates Fas from 3T3/A31 cells. RIP, a death domain containing protein, was co-precipitated with Fas. Optimal dilutions to be determined by the researcher. Positive Antigen Control: F4209: 3T3/A31 Cell Lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Use 20ug per lane for minigels. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice