F0019-62-ML650
Fas (CD95, APO-1) (MaxLight 650)
Size
£1,081.00
- SKU:
- F0019-62-ML650
- Purity:
- Purified by Protein G affinity chromatography from culture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant protein containing the cytoplasmic domain of mouse Fas
- Clone:
- 0.T.44 (7C10)
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Apoptosis or programmed-cell death is a physiological process essential for the normal development and maintenance of homeostasis in many organisms. This “cellular suicide” can be mediated by the Fas antigen (CD95, APO1), a cell-surface glycoprotein, 40-50kD, that belongs to the nerve growth factor/tumor necrosis factor (TNF) receptor family. Mice carrying the lymphoproliferation (lpr) mutation, which develop lymphoadenopathy and suffer from a systematic lupus erythematosus-like autoimmune disease, have defects in the Fas gene. Fas is expressed in several tissues including blood, where it expression is upregulated on activated T and B lymphocytes and natural killer cells. Binding of the Fas antigen by agonistic anti-Fas antibody has been shown to induce apoptosis. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice