F0019-62-BIOTIN
Fas (CD95, APO-1) (Biotin)
Size
£1,081.00
- SKU:
- F0019-62-BIOTIN
- Purity:
- Purified by Protein G affinity chromatography from culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant protein containing the cytoplasmic domain of mouse Fas
- Clone:
- 0.T.44 (7C10)
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
- Extra Details:
- Apoptosis or programmed-cell death is a physiological process essential for the normal development and maintenance of homeostasis in many organisms. This “cellular suicide” can be mediated by the Fas antigen (CD95, APO1), a cell-surface glycoprotein, 40-50kD, that belongs to the nerve growth factor/tumor necrosis factor (TNF) receptor family. Mice carrying the lymphoproliferation (lpr) mutation, which develop lymphoadenopathy and suffer from a systematic lupus erythematosus-like autoimmune disease, have defects in the Fas gene. Fas is expressed in several tissues including blood, where it expression is upregulated on activated T and B lymphocytes and natural killer cells. Binding of the Fas antigen by agonistic anti-Fas antibody has been shown to induce apoptosis. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice