F0019-61S

FAS (Tumor Necrosis Factor Receptor Superfamily Member 6, TNFRSF6, Apo-1 Antigen, Apoptosis-mediating Surface Antigen FAS, FASLG Receptor, CD95, APT1, FAS1, TNFRSF6)

Size

£628.00

SKU:: F0019-61S
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: HUT-78 human T cell lymphoma cell line
Clone:: LT95
Format:: Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Extra Details:: CD95 (Fas, APO-1), a 46kD transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular death domains, formation of the death-inducing signaling complex (DISC) and activation of caspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated at the DISC, in type II cells low concentration of caspase 8 activates pathway leading to the release of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokine responses. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1-12ug/ml Immunohistochemistry: paraffin Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.