F0019-60B
Fas (CD95, APO-1)
Size
£460.00
- SKU:
- F0019-60B
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant Fas protein
- Clone:
- 1.V.2A
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
- Extra Details:
- Apoptosis or programmed-cell death is a physiological process essential for the normal development and maintenance of homeostasis in many organisms. This “cellular suicide” can be mediated by the Fas antigen (CD95, APO1), a cell-surface glycoprotein, 40-50kD, that belongs to the nerve growth factor/tumor necrosis factor (TNF) receptor family. Mice carrying the lymphoproliferation (lpr) mutation, which develop lymphoadenopathy and suffer from a systematic lupus erythematosus-like autoimmune disease, have defects in the Fas gene. Fas is expressed in several tissues including blood, where it expression is upregulated on activated T and B lymphocytes and natural killer cells. Binding of the Fas antigen by agonistic anti-Fas antibody has been shown to induce apoptosis. Applications: Suitable for use in Immunoprecipitation, ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Immunoprecipitation: 2-5ug/10e6 cells Western Blot: 1:500 ELISA: 1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice