F0019-55B
FAK (Focal Adhesion Associated Protein Tyrosine Kinase, BC3) (Agarose)
Size
£891.00
- SKU:
- F0019-55B
- Application:
- IP
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- GST fusion protein corresponding to residues 1-423 of human FAK.
- Clone:
- 4.47
- Format:
- Covalently linked to 200ml of protein G agarose beads and provided as a 50% gel slurry suspended in PBS, 0.05% sodium azide for a total volume of 400ml. Liquid suspension.
- Extra Details:
- This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. At least four transcript variants encoding four different isoforms have been found for this gene, but the full-length natures of only two of them have been determined. Applications: Suitable for Immunoprecipitation and Affinity Purification. Other applications not tested. Recommended Dilution: Immunoprecipitation: 5-10ug (10-20ul) of this lot of antibody-agarose immunoprecipitated FAK from a mouse 3T3/A31 RIPA cell lysate, as confirmed by subsequent immunoblot analysis using anti-FAK. Storage and Stability: Stable for 2 years at 4°C from date of shipment. It is recommended to wash the agarose beads with appropriate buffer prior to use to remove sodium azide.
- Shipping Conditions:
- Blue Ice