F0019-50M
Factor XIIIa (Coagulation Factor XIII A Chain, Coagulation Factor XIIIa, Coagulation Factor XIII A1 Polypeptide, F13A, F13A1, bA525O21.1, Fibrinoligase, Protein-glutamine gamma-glutamyltransferase A Chain, Transglutaminase A Chain, TGase) (Not for Export EU)
Size
£594.00
- SKU:
- F0019-50M
- Purity:
- Serum.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Highly purified human Factor XIII-A.
- Format:
- Supplied as a lyophilized powder. Preservative free. Reconstitute with 1ml sterile ddH2O.
- Extra Details:
- Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as plasma carrier molecules. Platelet factor XIII is comprised only of 2 A subunits, which are identical to those of plasma origin. Upon cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIIIa, as platelet factor XIII. This enzyme acts as a transglutaminase to catalyze the formation of amma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. It also crosslinks alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion. Applications: Suitable for use in ELISA, Immunoelectrophoresis, Immunoelectrodiffusion and Double Radial Immunodiffusion. Other applications not tested. Recommended Dilutions: Immunoelectrophoresis (agarose plates): 120ul antiserum per 2ul plasma or equivalent Double Radial Immunodiffusion: Use a rosette arrangement with 10ul antiserum in 3mm diameter center well and 2ul plasma samples (neat and serially diluted) in 2mm diameter peripheral wells. Electroimmunodiffusion: 1-2% antiserum depending on the test arrangement. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice