Factor IX

Size

£633.00

SKU:: F0017-08
Purity:: Purified by affinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: Human Factor IX purified from plasma.
Format:: Supplied as a liquid in 10mM HEPES, pH 7.4, 0.15M sodium chloride, 50% glycerol.
Extra Details:: Factor IX (FIX, Christmas Factor) is a vitamin K-dependent glycoprotein produced in the liver. Plasma concentration of FIX is normally around 5ug/ml (87nM) in plasma. The biological importance of FIX is demonstrated in Haemophilia B (Christmas disease). This is an X-linked congenital bleeding disease resulting from a quantitative (low activity and low antigen) or qualitative (low activity and normal antigen) defect in FIX function. In its proenzyme or zymogen form, FIX is a single chain molecule of 55kD. It contains two EGF-like domains and an amino-terminal domain containing 12-g-carboxy-glutamic acid (Gla) residues. These Gla residues allow FIX to bind divalent metal ions and participate in calcium-dependent binding interactions. Applications: Suitable for use as ELISA, Neutralization and other solid phase immunoassays for Factor IX and its activation products. Other applications not tested. Recommended Dilutions: ELISA: Suitable for use as the capture antibody. One Bethesda unit/ml is defined as the amount of inhibitor that resulted in 50% residual F. IX acitivty after 2 hours at 37°C (Kasper CK et al., Thromb Diath Haemorrh 34:869, 1975). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice